Murias Laboratories, LLC specializes in bulk production of human proteins expressed in human cell lines. Thus, the proteins are appropriately post-translationally modified for use in research monitoring human cell signaling events.
We also express mutant and customized proteins in human cells as well as in conventional bacterial and insect cell systems. Additionally, we offer generation of stable cell lines for your research.
Contact Murias Labs to supply the proteins needed for your research at info@muriaslabs.com.
Murias Labs offers recombinant protein production services with skilled staff members each having over 15 years experience in expression and purification of proteins using a wide variety of systems. We also have extensive experience constructing expression plasmids. Our customers are provided with high quality recombinant proteins expressed in E.coli, insect cells or human cells. Starting with the amino acid sequence or gene ID and information about affinity tags and protein quantity required, we will initiate the project to deliver purified proteins for the client’s research at an extremely competitive price.
Example of typical cost and timeline for expression and purification from a bacterial cytosolic fraction.
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PHASE I:
GENE SYNTHESIS: Gene design and synthesis
Estimated Time: 3-4 weeks
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PHASE II:
SUBCLONING Amplification/isolation of hte gene of interest and subcloning into a bacterial expression vector
Verification of the authenticity of hte subcloned gene by sequence analysis
Estimated Time: 1-2 weeks
Price: $750.00
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PHASE III:
EXPRESSION and PURIFICATION Transformation of the recombinant constructs into high-efficiency expression bacterial strain
Expression and affinity prurification
Quality control by SDS-PAGE, silver stain and immunoblot analysis
Estimated Time: 2 - 4 weeks
Deliverables: 5 mg of purified, soluble, affinity-tagged protein
Quality control data
Price: $1,000.00
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PHASE IV (OPTIONAL):
TAG REMOVAL Efficient tag removal
High purity end product
Estimated Time: 1 week
Price: $500.00
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PRICE OF SUBSEQUENT
5 mg PREPARATIONS: $1,000.00
(excluding affinity-tag
removal)
